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Immunofluorescence Cells were washed with PBS at the end of treatments, if applicable, and then fixed with 4% paraformaldehyde for 15 minutes
Namely, it is well established that GSTT1 enzyme is involved in bioactivation, rather than detoxification of several bifunctional alkylating agents, present in environmental pollution and certain occupational hazards 45
This fibrotic response was substantially attenuated in the palmatine-treated groups, evidenced by reduced collagen accumulation and partial restoration of hepatic lobule structure
Protein concentration in the supernatant was determined using the bicinchoninic acid assay kit (Pierce, Rockford, IL, USA)
Studies have relayed it may help maintain cardiovascular function, support cognitive health and reduce oxidative stress, contributing to healthy aging